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More About This Title Understanding Light Microscopy
English
Introduces readers to the enlightening world of the modern light microscope
There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades.
Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more.
- Gives a didactic introduction to the light microscope
- Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility
- Features full-colour illustrations and workable practical protocols
Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.
English
JEREMY SANDERSON is a Bio-Imaging specialist at the Mammalian Genetics Unit, MRC Harwell Institute, in the UK. He is Chartered Scientist and a Fellow of the Royal Microscopical Society and has served both on the Executive Council and the LM Section Committee.
English
About the Author
Acknowledgements
Glossary and definitions
About the Companion Website
Notes
Introduction
Chapter 1 Our eyes and the microscope
Chapter 2 Light
Chapter 3 Basic microscope optics
Chapter 4 Microscope design
Chapter 5 Ergonomics
Chapter 6 Optical aberrations of the microscope
Chapter 7 The microscope objective
Chapter 8 Condensers & eyepieces
Chapter 9 Illumination in the microscope
Chapter 10 Diffraction and image formation in microscopy
Chapter 11 Contrast generation & enhancement
Chapter 12 Reflected-light microscopy
Chapter 13 Polarised-light microscopy: part 1 - theory
Chapter 14 Polarised-light microscopy: part 2 - applied
Chapter 15 Fluorescence microscopy
Chapter 16 Fluorophores & fluorescent proteins
Chapter 17 Optical sectioning & confocal microscopy
Chapter 18 Operating the confocal microscope
Chapter 19 Light-sheet microscopy
Chapter 20 Bleed-through & Spectral unmixing
Chapter 21 Deconvolution
Chapter 22 Multi-photon microscopy
Chapter 23 Total Internal Reflection Fluorescence microscopy
Chapter 24 FRAP and FRET
Chapter 25 Colocalisation
Chapter 26 Super-resolution fluorescence microscopy
Chapter 27 - Choosing a microscope platform and Core Imaging Facilities
Chapter 28 Biological Specimen Preparation
Chapter 29 Materials Specimen Preparation
Chapter 30 Recording the image: part 1 - theory
Chapter 31 Recording the image: part 2 - applied
Appendices
1. Buying and tendering for a light microscope
2. Trouble-shooting poor image quality
3. The Michel-Lévy Interference chart
4. Cleaning and Maintenance
5. Microscope Manufacturer contacts
6. Brief historical notes
7. Timeline
Index
