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- Wiley
More About This Title Culture of Animal Cells, 3rd Edition
English
New Topics covered include:
the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome paintingcell interactions in cell culturenew methods for separating cellsnew or refined methods for accessing cytotoxicity, viability, and mutagenicityexperimental details for culture of specialized cells types not covered in previous editionsnew or refined techniques for visualizing clues, including time-lapse photography and confocal microscopyThe revised and expanded third edition offers the following features:
over 350 new reference to the primary literaturean international list of cell banksan international listing of reagants and commercial suppliesa subject indexa glossaryAlso available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est.
From the reviews:
"I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology
"It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports
English
Design and Layout of the Laboratory.
Equipping the Laboratory.
Aseptic Technique.
Laboratory Safety and Biohazards.
The Culture Environment: Substrate, Gas Phase, Medium and Temperature.
Preparation and Sterilization.
Disaggregation of the Tissue and Primary Culture.
Maintenance of the Culture: Cell Lines.
Cloning and Selection of Specific Cell Types.
Physical Methods of Cell Separation.
Characterization.
Induction of Differentiation.
The Transformed Phenotype.
Contamination.
Instability, Variation, and Preservation.
Quantitation and Experimental Design.
Measurement of Viability and Cytotoxicity.
Culture of Specific Cell Types.
Culture of Tumor Tissue.
Three-Dimensional Culture Sytems.
Specialized Techniques.
Reagent Appendix.
Glossary.
References.
Indexes.
English
"It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" —Cell Biology International Reports
