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More About This Title Practical Flow Cytometry in Haematology - 100Worked Examples
English
The analysis of blood, bone marrow and tissue fluid specimens requires a multi-faceted approach with the integration of scientific data from a number of disciplines. No single discipline can operate in isolation or errors will occur. Flow cytometry is in a privileged position in that it can provide rapid analysis of specimens and it is often the first definitive investigation to produce results and help formulate a working diagnosis.
This companion text to Practical Flow Cytometry in Haematology Diagnosis contains 100 worked examples drawn from real clinical cases presenting to the authors’ institution. Cases are illustrated with peripheral blood and bone marrow cytology, tissue pathology and cytogenetic and molecular data, which are integrated to generate, where appropriate, a diagnosis based on the WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. The spectrum of clinical cases includes adult and paediatric patients, and both neoplastic and reactive disorders. The cases appear in no particular order to challenge the reader to make their own diagnosis.
The reader will review May−Grünwald−Giemsa (MGG)-stained films of peripheral blood and bone marrow aspirates presented alongside flow cytometric data and haematoxylin and eosin (H&E)-stained bone marrow and other tissue biopsy sections. Immunohistochemistry is used to further clarify the tissue lineage and cell differentiation. Cytogenetic studies using metaphase preparations are used to identify translocations and chromosome gains and losses whilst interphase fluorescence in situ hybridisation (FISH) studies and polymerase chain reaction (PCR) are used to identify gene fusions, gene rearrangements and deletions. Each case concludes with a discussion of the features that are important to making a diagnosis. The cases are also listed according to disease classification in the appendix so that the text can also be used as a reference.
Practical Flow Cytometry in Haematology: 100 Worked Examples:
- Provides a practical, example-based resource for flow cytometry
- Demonstrates how flow cytometry results should be interpreted and applied to optimize patient care
- Includes both malignant and benign conditions
- Can be used in conjunction with Practical Flow Cytometry in Haematology Diagnosis, by the same author team (ISBN 9780470671207)
Practical Flow Cytometry in Haematology: 100 Worked Examples is ideal for practicing haematologists and histopathologists with an interest in haematopathology, but particularly directed at trainee haematologists and scientists preparing for FRCPath and related examinations.
English
Mike Leach FRCP, FRCPath
Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK
Mark Drummond PhD, FRCPath Consultant Haematologist and Honorary Senior Lecturer, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK
Allyson Doig MSc, FIBMS Haemato-Oncology Laboratory Manager, Haematology Laboratories Gartnavel General Hospital, Glasgow, UK
Pam McKay FRCP FRCPath Consultant Haematologist, Haematology Laboratories and West of Scotland Cancer Centre Gartnavel General Hospital, Glasgow, UK
Bob Jackson MB MRCPI FRCPath Haematopathology Laboratories, Southern General Hospital, Glasgow, UK
Barbara J. Bain MB BS, FRACP, FRCPath, Professor of Diagnostic Haematology, St Mary’s Hospital Campus of Imperial College Faculty of Medicine, London and Honorary Consultant Haematologist, St Mary’s Hospital, London, UK
English
Preface, vii
Acknowledgement, ix
List of Abbreviations, xi
Technical Notes, xv
Laboratory Values, xix
Case 1 1
Case 2 6
Case 3 11
Case 4 15
Case 5 18
Case 6 21
Case 7 24
Case 8 27
Case 9 31
Case 10 35
Case 11 39
Case 12 43
Case 13 46
Case 14 50
Case 15 54
Case 16 59
Case 17 62
Case 18 65
Case 19 68
Case 20 70
Case 21 74
Case 22 77
Case 23 80
Case 24 82
Case 25 87
Case 26 90
Case 27 93
Case 28 95
Case 29 100
Case 30 104
Case 31 106
Case 32 110
Case 33 114
Case 34 117
Case 35 122
Case 36 126
Case 37 129
Case 38 132
Case 39 136
Case 40 140
Case 41 143
Case 42 146
Case 43 151
Case 44 154
Case 45 159
Case 46 163
Case 47 166
Case 48 168
Case 49 172
Case 50 177
Case 51 180
Case 52 183
Case 53 186
Case 54 189
Case 55 193
Case 56 196
Case 57 201
Case 58 206
Case 59 210
Case 60 213
Case 61 216
Case 62 218
Case 63 224
Case 64 227
Case 65 232
Case 66 236
Case 67 240
Case 68 244
Case 69 249
Case 70 253
Case 71 256
Case 72 260
Case 73 266
Case 74 269
Case 75 274
Case 76 276
Case 77 281
Case 78 284
Case 79 289
Case 80 292
Case 81 297
Case 82 300
Case 83 306
Case 84 310
Case 85 315
Case 86 319
Case 87 321
Case 88 325
Case 89 327
Case 90 330
Case 91 334
Case 92 338
Case 93 342
Case 94 347
Case 95 351
Case 96 355
Case 97 359
Case 98 365
Case 99 370
Case 100 375
Antibodies Used in Immunohistochemistry Studies, 381
Flow Cytometry Antibodies, 386
Molecular Terminology, 389
Classification of Cases According to Diagnosis, 390
Index, 391
